The present article aims to assess psychometric characteristics of a Russian version of the Big Five Inventory-2 (BFI-2). This questionnaire measures five basic personality domains, as well as three facets per domain. We collected data from an Internet sample comprised of 1,787 people (31.9% of men) aged from 14 to 54 years (M = 26.31; SD = 7.76). The study covered over ten regions of the Russian Federation. The factorial structure of the BFI-2 was examined using the principal component analysis, confirmatory factor analysis and random intercept exploratory factor analysis. The five-factor structure of the BFI-2 was confirmed both at the domain and facet levels. Strict measurement invariance was obtained across sex, which makes it possible to compare raw scores of the questionnaire when assessing sex differences. Sex differences obtained in this study were consistent with those published in the extant literature. Across the BFI-2 subscales, internal consistency measured by the Cronbach’s alpha and McDonald’s omega ranged from satisfactory to excellent. The Ferguson’s delta (adapted by M. Hankins) was high which shows that the Russian BFI-2 can distinguish individuals with various manifestations of a domain or facet. To summarize, the Russian version of the BFI-2 represents a reliable and valid tool for measuring the basic traits of personality.