The article presents the Russian adaptation of the Ambivalent Sexism Scale by P. Glick and S. Fisk. This tool measures not only hostile (a tendency to negatively appraise women that violate traditional gender roles), but also benevolent (a tendency to positively appraise women, who comply with the traditional gender roles) sexism. In the approbation of the scale 1624 Russian citizens took part. The full version of the scale demonstrated satisfactory fit to the empirical data, due to more covariance between the items. On the basis of the found covariance between the items, a short version was formed. The short version of the scale comprised 12 items (6 for the subscale of the hostile sexism and 6 for the subscale of the benevolent sexism), it demonstrated a good fit to the empirical data (χ² = 332.147, df = 53, RMSEA = 0.057, CFI = 0.960, TLI = 0.950). Multigroup analysis demonstrated full structural and factorial equivalence with the use of the short version of the scale on the sample of men and women, as well as heterosexuals and people with non-heterosexual identification. Social-demographical differences were found with the use of the short version of the scale. Men and heterosexuals in a stronger degree, than women and non-heterosexuals, demonstrate hostile and benevolent sexism, people older than 30 in a stronger degree show benevolent sexism, than younger respondents. In the whole the presented variant of the short version of the scale demonstrates good psychometric indices and can be used for further studies as a reliable and valid instrument.